Application Note no.H1

Title : Amitriptyline and Nortriptyline in standard solution 

Summary : Amitriptyline and nortriptyline are tricyclic antidepressants originally designed for use in the treatment of depression.

Nortriptyline is a metabolite of amitriptyline as well as a drug in its own right.

Sample : 50ppm standard solution of Amitriptyline and Nortriptyline

HPLC system : DanChrom Model A4

Separation column : Reprospher C8 150 x 4.6 mm

Mobile phase : 40% ACN / 60% 0.01M sodium dihydrogen Phosphate buffer

Flow Rate : 1ml/min 

Detection : UV240 nm